Orange found over grapefruit, lemon and lime favorable for diet and teeth in protein concentration, sugar and pH tests
Abstract: Written by Austen Thelen
Edited by: Christina Cherian and Deanna Lanier
The human diet consists of a variety of foods, and
citrus fruits are considered an important component because they contain a
large amount of vitamin C (Hay, 1998). Humans lack an enzyme that
manufactures this vitamin, so we must obtain it through diet (Hay, 1998).
Although citrus fruits are a good source of vitamin C for a balanced diet and
the immune system, they contain organic acids (citric, malic,
and lactic acids) that can contribute to the weakening of tooth enamel (Dental Websmith, 2001-2003).
Discussion: Written by Christina Cherian
Edited by: Bailey Schroeder and Austen Thelen
A study from the Division of Restorative Dentistry showed that the erosion of tooth enamel is influenced by dietary acids, pH and acid concentration (Hughes, et al, 2000). The lower pHs initiate more damage to tooth enamel (Dental Websmith, 2001-2003). The purpose of our research was to find which of four citrus fruits ñ grapefruit, lemon, lime and orange ñ was the best for the human diet and least harmful to the teeth. We decided that the citrus fruit with the greater variety of dietary (reducing) sugars, including monosaccharides, the highest concentration of proteins and the highest pH would be the most beneficial to eat and the least damaging to tooth enamel. This was determined because monosaccharides are easier to digest because of their simplicity, and that higher concentrations of proteins are digested to provide higher quantities of amino acids, which are used by the body. Our prediction was that the orange had the greatest variety of dietary (reducing) sugars, the highest level of proteins and was the least acidic. The orange therefore would be the most beneficial to the human diet.
We thought this because human taste buds are able to distinguish between sweet and bitter tastes and the orange tasted the sweetest, therefore it would be the one with the highest pH.
During the course of our investigation we performed three sets of experiments to determine which was the best fruit. The first set were assays to determine the presence of various sugars in the fruits. Benedictís Test was performed to determine if reducing sugars (sugars that are able to reduce ions) were present in the fruits. The results of this test revealed that grapefruit, lime and orange were positive for reducing sugars while lemon was negative. Fructose, the control, also resulted in a positive outcome. It is known that there are reducing sugars in lemons, but they can have as little as one percent within their contents where other citrus fruits such as oranges can have up to nine percent (Salunkhe, 1995). Reducing sugars are most likely present in lemons in such a low amount that any precipitate was not recognizable during the experiment.
Next, we performed Barfoedís Test to distinguish the fruits that contained monosaccharides from the ones that did not. The first time that Barfoedís Test was completed it was invalid. Fructose is a known monosaccharide and should have reacted positively to form a red participate, however fructose did not react in the first trial. We believe that this trial did not react correctly because the water was not hot enough. The second trial of Barfoedís Test produced a positive result for fructose. Grapefruits and oranges tested positive for monosaccharides and lemons and limes tested negative. Though only grapefruits and oranges tested positive for monosaccharides, all of the fruits may contain di and polysaccharides. This is because Barfoedís Test can only determine if there are monosaccharides present, it cannot confirm or refute the presence of di- or polysaccharides. Also, lemons and limes may contain monosaccharides, but in amounts too low to produce a positive result. Monosaccharides are more beneficial to consume because they are less complex than polysaccharides and are therefore easier to digest. Since oranges and grapefruits contain monosaccharides they are more favorable.
The Iodine Test was performed to determine if any of the fruits contained starch. After completion of the Iodine Test the results suggested that none of the four citrus fruits contained starch. The Iodine Test concluded that none of the fruits contained starch. Since starch is long chained polysaccharide it is harder to digest within the body, its absence in fruits makes the fruit easier to digest.
During the second set of experiments we performed our pH test and the absorption spectrum experiment. The pH levels showed that the lemon was the most acidic with an average pH of 2.75, followed by the lime: 2.88, grapefruit: 3.65, and then the orange at 4.35. Since studies show that the lower the pH the greater the erosion of tooth enamel, this supports our hypothesis that the orange is the least damaging to teeth (Dental Websmith, 2001-2003). The orange is followed closely by grapefruit and then by lime and lemon, which cause higher levels of tooth enamel erosion.
The absorption spectrum experiment showed which
wavelengths of light the pigments in the fruit juice absorbed. Grapefruit
had an absorption peak at 400 nm with its lowest point at 700 nm.
The Bradford Assay tested the total protein concentration of each fruit. To find the protein values for each fruit to compare to one another we chose to use 20 ml of the diluted fruit juices. For the first trial the protein values found for all the fruits was 20 mg at an absorbance of 0.075 for grapefruits and lemons, 0.05 for limes and 0.025 for oranges. In the second trial grapefruitís absorbency was 0.81, limeís 0.025, lemonís 0.075 and orangeís was 0, again for 20 ml. Because there is no visible pattern between the fruit spectrums and the absorbance values are not consistent between the two trials, no solid conclusion can be reached to determine which one has the greater amount of protein. The results of the Bradford Assay were inconclusive.
After thoroughly conducting all of the experiments and a very detailed
investigation analyzing all of the results, our hypothesis was supported by the
sugar and pH tests.
There are several
sources of error that may have occurred with in our experiments. One of
them may have been that the citrus fruits used may have varied in age. As
fruit matures, the sugar content is known to rise, while acidity level
decreases (Hay, 1998). Though we tried to pick out fruits with
approximately the same size, shape, color and firmness for each set of
experiments, we did use new fruits for each set so the maturity level may have
differed. We also found research showing that fruit composition is
affected by factors such as growing conditions, variety, and climate (Hay,
1998). When Meijers was out of a particular
fruit we were forced to go to Farmer Jackís to purchase it. Since stores
buy their fruits from companies, such as Del Monte, Outspan
and Sunkist that grow the fruits it is possible that these stores did not
purchase their fruits from the same company. Also, because companies grow
their fruit in multiple locations it is most likely that the fruits we used
were grown in different locations. The use of fruits grown in different
locations for each set of experiments could have caused a discrepancy in the
fruit juice composition, therefore providing us with varying results.
Another source of error may have been human error that occurred while
conducting the numerous trials of our experiments. The experiments we
performed required close detail to the amount of solutions, such as our juices,
put into them and to time restrictions within the experiments. Many of
the experiments performed required small quantities of solutions are were very
sensitive to the amount added, such as the
Figure 1: Benedictís Test
for Fructose, Grapefruit, Lemon,