Mariam Sticklen

Mariam B. Sticklen, PhD

Professor

Biofuel & Biopharmaceutical Crop Genetic Engineering Lab.

362 PSSB

Department of Crop and Soil Sciences

Michigan State University

East Lansing, MI 48824

Tel: 517-230-2929

E-mail: stickle1@msu.edu

Web site: http://www.msu.edu/~stickle1

North America Regional Editor

Journal of Biobased Material and Bioenergy (JBMBE)

http://www.aspbs.com/jbmbe/

Editorial Board Member Biofuels,

The Society of Chemical Industry (SCI)'s Biofuels, Bioproducts & Biorefining

Journal

http://www.soci.org/SCI/publications/biofpr.jsp

Editorial Board Member

International Journal of Agronomy

http://www.hindawi.com/journals/ija/

Citizenship: USA

Years of full-time Michigan State University faculty service: 20 years

Years of full-time Ohio State University faculty service: 4 years

Years of full-time Clemson University faculty service: 1 year

Highest Degree Obtained: Ph.D. (June 1981), The Ohio State University, Cols, OH.

EMPLOYMENT AND CAREER ACTIVITIES:

• 2000 to Present Professor, Department of Crop and Soil Sci., MSU.

• 1995 to 2000 Assoc. Prof., Department of Crop and Soil Sci., MSU.

• 1991 to 1995: PI and Res. Director, ABSP (A $12 million grant), MSU

• 1987 to 1995 Assistant Professor, MSU

• 1983 to 1987 Instructor, Agron. Dept. Ohio State University

• 1981-1982 Assist. Prof. (non-tenure stream), Clemson Univ., SC.

LATEST ADVISORY ROLE

2005 – 2006 U.S. National Academy of Sciences, on Genome To Life (GTL) for Biofuel Energy

2002 – 2004 U. S. National Academy of Sciences, on Bioconfinement of Genetically Engineered Organisms.

2008 - Present Member of the Science Advisory Board Report on Careers in Life Science Industry

2008-Present U.S. National Academies NRC on State of Ohio’s Industrial Biotechnology for Creation of Jobs

BOARD OF TRUSTEES APPOINTMENTS:

• 1999 to 2000

Chairperson, Nomination Committee, Board of Trustees of International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). ICRISAT is one of the CGIAR-funded ($35 million) international research centers.

• 1999 to 2000

Member, Executive Committee, Board of Trustees of International Crops Research Institute for the Semi-Arid Tropics (ICRISAT).

• 1994 to 2000 (two terms)

Member, Board of Trustees, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in India and Africa.

• 1994 to 1996

Member, Board of Trustees of Consultative Group on International Agricultural Research (CGIAR) in Washington, DC.

Publications, Patents, Invited Speeches in Different Countries, and Persons Supervised

Time Period

Invited Speeches

Patents

Books

Referred & Other Publications

Supervised

Since at MSU

(1987)

50 invited speeches

in 25 different countries

9 issued

3 (published by U.S. National Academies Press,

Springer-Verlag, & Ann Arbor Press.

Over 500

Over 160 (54 long-term) scientists

EXPERTISE AND AREAS OF INTERESTS

Professor Sticklen’s research bridges basic to applied research on addressing the production of industrial biotechnological molecules (proteins and polymers) in biomass of feedstock crops for a sustainable biofuel and other biobased economy. She develops and uses bioconfinement methods of genetically engineered feedstock crops to avoid public concerns.

She uses feedstock genetic transformation to produce these materials She develops and uses genetic modification (GM) technologies that have no or very minimum concerns among the public. Among these, she has developed corn chloroplast transgenesis system for production GM corn plants which have low-risk or no risk associated with their pollen flow in the field because corn chloroplast genome is only maternally inherited.

She also produces the industrially important molecules through a GM technology that produces the molecules only in feedstock crop leaves and stems (not in seeds, pollens or roots).

The specific biobased molecules that she has produced in corn leaves and stalks include: (1) Heterologous biodegradable plastic, polyhydroxybutyrate, (2) Heterologous microbial endoglucanase (see Figure 1 below), (3) Heterologous microbial exoglucanase, and (4) Production of a South African rumen anaerobic microbe cellobiase enzyme in corn biomass (not in seeds, pollens or roots; see Figure 2 below from Ransom et al, in progress). She produces all these industrial molecules successfully in crops to replace the same molecules that are at present expensively produced in microbial fomenters.

She has produced all of the above molecules in five different plant cell compartments of corn biomass. These cell compartments include chloroplast, endoplasmic reticulum, apoplast, vacuole and mitochondria. The reason for multi-targeting of these molecules include; (1) to improve the protein folding and therefore the biological activity of the heterologous proteins because certain of these sub-cellular compartments (for example endoplasmic reticulum) have molecular chaperons capable of assisting an improved protein folding and activity. (2) Also, multi-subcellular targeting of heterologous molecules is expected to increase the level of heterologous molecule production.

Another area of research of Dr. Sticklen is down regulation of lignin biosynthesis pathway enzymes to modify lignin configuration and content. Lignin down regulation in biofuel model crops such as alfalfa have shown less or no needs for pretreatment processes. She has used two technologies to down regulate lignin biosynthesis enzymes in corn based on the publically available mapped corn genome. These two technologies include (1) RNAi, and (2) gene antisense. To date, down regulation of two of the major corn lignin biosynthesis pathway enzymes has proven to ease the deconstruction of corn cell walls without much needs for harsh and expensive pretreatment processes. It is encouraging to see that the lignin down-regulated transgenic corn plants are all morphologically normal and produce viable seeds (patent pending).

Invited Speeches/Panelist Around the Globe:

Feb 2008 Invited Proposal Writing Workshop Panelist. CPBR Annual Conference, Washington DC

Feb 2008 Forum Panelist. DOE-Development of Renewable Energy Projects at DOE, 2008, Washington, DC

Feb 2008 Invited poster presenter to the CPBR Congressional Reception, Feb 12^th Evening, Capital Hill, Washington, DC.

Mar 2008 US-UK Bioenergy Workshop, Cambridge-MIT Partnership, and Cambridge, UK (cancelled by organizer)

Apr 2008 American Chemical Society, 235^th National Meeting, April 5-10, 2008. "Modifying the corn genome to meet the US biofuel agenda" New Orleans.

Apr 2008 Southern Mississippi University. Spring Speaker Scholar Series. April 10, 2008, Hattiesburg, Production of a natural human saliva anti-HIV in plants.

May 2008 30^th Symp. on Biofuels and Chemicals, May 6, 2008. New Orleans

June 2008 Corn Utilization and Technology Conference of Natl Corn Marketing Association, Kansas City, Mo.

Jun 2008 Society of In Vitro Biology Conf. “Modifying the corn genome to increase its biomass biofuel production.” Tucson, AZ

Apr 2007 National Renewable Energy Laboratory

Nov 2007 American Sugar Assoc: Sugar/Ethanol Conference “New possibilities for improving the yield of fermentable sugars from corn leaves and stalk for alcohol fuels.”

Jan 2007 Corn Marketing program of Michigan. Reducing the costs of biomass pretreatment via lignin down-regulation; an RNAi technology.

May 2007 Plenary Speaker and Panel Co-Chairperson. BIO. Boston: Food and Agriculture Track of the Energy Crops for Biofuel, May 9, 2007

May 2007 Panel Chairperson at BIO Boston; Industrial and Environmental Track: Energy Crops for Biofuel, May 8, 2007

May 2007 MSU Bioeconomy Seminar Series

Jul 2007 Greater Lansing Clean Cities Coalition, July 2007

Dec 2007 MSU CSS/HORT, Dec 2007

Jan 2006 CPBR Annual Conference, Jan., 2006. Maize chloroplast transgenesis.

Dec. 2006. Efficient production of microbial cellulase within recombinant maize biomass converts AFEX-pretreated corn stover, Genetic Engineering Center (BIOTECH), Bangkok, Thailand

Aug. 2006 Feedstock crop genetic engineering for biofuels. Oak Ridge National Research Laboratory, Oak Ridge, TN

May 2006. Converting rice straw into fermentable sugars for alcohol fuels, 28^th Symp. on Biofuels & Chem., Tennessee

May 2006. Production of Acidothermus cellulolyticus endoglucanase (E1) enzyme in transgenic rice. 28^th Symp. on Biofuels & Chem., Tennessee

Nov. 2006. Production of cellulases within crop biomass for biofuels. Bioeconomy Program. Michigan State University, East Lansing, MI

Feb. 2006. Cheap production of human wound healing protein in plants. Consortium for Plant Biotechnology Research Conference, Washington, DC

March, 2005 “Producing the Microbial Hydrolysis Enzymes within the Biomass Crops via Genetic Engineering” Department of Plant Sciences, Oxford University, Oxford, U.K.

Nov. 2005 Producing an anti-HIV drug, Human Secretory Leukocyte Protease Inhibitor in plants. HIV Research Center, Mayo Clinic, Rochester Minn.

Aug. 2005 Biomass, Biofuel, and Biopharma: Using biomass crops as biofactories for production of biomass conversion hydrolysis enzymes and an anti-HIV protein. Genetics Program. MSU

May, 2005 Production of third generation salt tolerance oat via Biolistic gun transfer of a barley Hva1 transgene. Chloroplast Symposium. Champaign, IL

May, 2005. “The Development of Transgenic Non-Food Biomass Crops for Renewable Energy and Cleaner Environment.” 27^th Symposium on Biotechnology for Fuels and Chemicals. Denver, CO.

March, 2005 Production of biofuel hydrolysis enzymes within the crop biomass. Dept of Agronomy Department. NC State University, Raleigh, NC.

April, 2005 Producing the biofuel-related industrial enzymes within the corn biomass. Second Annual World Congress on Industrial Biotechnology and Bioprocessing. Orlando, Fl

Oct. 2004 The United States National Academies Workshop on Global Challenges for Guiding and Managing Biological Technologies: “Opportunities to Improve Food Security and Human Nutrition: Coping with Challenges of Human Health in Developing Nations.” The U.S. Natl. Academies Bldg. Washington, DC. USA.

Sept. 2004 The Ukrainian National Academy of Sciences 2^nd Ukrainian Workshop on Biofuels and Biobased Industrial Material: Production of Biofuel Ethanol and PHB Biodegradable Plastic from Transgenic Biomass Crops. The Ukrainian natl. Academies Bldg. Kiev, Ukraine.

July 2004 “Evolution in development of safe GM crops: New molecular tools for biological confinements to protect human health and environmental integrity.” United States Department of Agriculture/Agricultural Research Center, Albany, CA, USA.

Dec. 2002 “Genetic engineering of corn for production of industrial polymers and enzymes.” Ankara University, Turkey.

April 2001 “Needs for improving crops of developing versus developed countries.” ICARDA, Syria.

April 1999 “Role of biotechnology in feeding the world population.” Ministry of Agriculture, New Delhi, India.

March 1999 “A transgene from DED resistant elm tree has induced disease resistance in turfgrass.” University of Toronto, Canada.

Feb. 1999 “Role of biotechnology in sustainable agriculture.” Sustainable Agriculture Program, Michigan State University, East Lansing, MI. USA.

Feb. 1999 “Meristem: A sustainable explant for genetic engineering of cereals.” Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.

Sept. 1998 “Novel system for genetic engineering of crops of Africa and India.” ICRISAT, Hyderabad, India.

July 1998 “Genetic improvement of turfgrass via genetic engineering.” INRA (similar to USDA), University of Caen, Normandy, France.

July 1998 “Meristem: A sustainable explant for genetic engineering of maize, oat, sorghum and wheat. John Innes Instit. Norwich, England.

Oct. 1997 “Herbicide and disease resistant creeping bentgrass via genetic engineering.” ASA, Anaheim, CA.

Sept. 1997 “Genetic engineering of cereal crops.” Hyderabad, India.

Sept. 1997 “Meristem: A sustainable explant for genetic engineering of maize.” The Rockefeller Foundation Annual Conference in biotechnology. Koala Lumpur, Malaysia.

Feb. 1996 “Genetic engineering of ICRISAT crops.” Hyderabad, India.

Feb. 1996 “Improving sorghum via genetic engineering.” National Center for Sorghum Research. Rajendranagar, India.

April 1996 “Production of insect resistant transgenic plants and their use in integrated pest management: Present status and future challenges.” National Soc. of Plant Sciences. Haryana Agric. Univ. Hisar, India.

May 1996 “A simple technique for genetic engineering of rice plants.” 3rd. Asia Pacific Biotechnology Congress and the Philippines Soc. for Microbial. Manila, The Philippines.

Sept. 1995 “Present and future of development on transgenic plants with Bacillus thuringiensis genes.” Bogotá, Columbia.

June 1994 “Introduction of proteinase inhibitor II gene in Basmati rice via microprojectile bombardment. Congress on Cell and Tissue Culture. Research Triangle, NC.

May 1994 “Transformation studies on rice (Oryza sativa L.) with potato proteinase inhibitor gene for insect resistance.” Seventh Meeting of International Program on Rice Biotechnology. Rockefeller Foundation Sponsored Conference. Bali, Indonesia.

May 1994 “Genetic transformation of rice (Oryza sativa L.) using a modified Bacillus thuringiensis Cry1A(b) gene for insect resistance. Seventh Meeting of International Program on Rice Biotechnology.” Rockefeller Foundation Sponsored Conference. Bali, Indonesia

Feb. 1994 “Genetic engineering of corn via a novel genotype-independent system.” International Center for Genetic Engineering, Bangkok, Thailand.

Jan. 1994 “The ABSP research on maize with AGERI.” Agricultural Genetic Engineering Research Institute. Giza, Egypt.

Oct. 1993 “Vegetative and reproductive plasticity of corn in vitro.” International Course in Plant Biotechnology. Havana, Cuba.

Aug. 1993 “The ABSP research.” University of Costa Rica. Costa Rica.

Feb. 1993 “Cloning of PR genes and genetic engineering of rice.” Thailand Agricultural Research Center. Chiang Mai. Thailand.

Feb. 1993 “Genetic engineering of monocot and dicot plants for crop improvement.” University of Dhaka, Bangladesh.

Jan. 1993 “Genetic engineering of creeping bentgrass.” National Agricultural Genetic Engineering Laboratory, Cairo, Egypt.

Dec. 1993 “In vitro plasticity in corn.” CIMMYT, Mexico.

Aug. 1992 “Stable expression of foreign genes in greenhouse grown monocot and dicot plants.” University of Heibi, China.

Aug. 1992 “Genetic engineering of monocot and dicot plants for crop improvement.” Central Res. Inst. for Food Crops (CRIFC), Bogor, Indonesia.

Sept. 1992 “Adaptation of plants to stressful environments while preserving productivity.” Symposium on Plant Stress in Tropical Environment. Kona, Hawaii, U.S.A.

May 1992 “New approaches for transferring biotechnology to third world countries.” ISU Showcase and Life Science Grand Opening, Ames, IA, U.S.A.

Feb. 1992 “Genetic engineering of cereal crops for pest resistance.” University of Cairo, Egypt.

Feb. 1992 “Mini-gene construction and genetic engineering of crops for pest resistance.” ILRAD, Kenya.

Oct. 1991 “Genetic engineering of rice using the microprojectile bombardment system.” Fifth Ann. Intl. Program on Rice Biotech. Tucson, AZ. U.S.A.

April 1990 “Transformation of rice using a microprojectile Biolistic™ device.” Biotechnology Section of Advanced Research Laboratory, Hitachi Ltd. Hatoyama Sitama, Japan.

Sept. 1990 “Genetic engineering of rice and corn using protoplast electroporation and the Biolistic™ tissue bombardment systems.” Plant Genetic Systems. Gent, Belgium.

April 1990 “State of the art in gene transfer to cereal crops.” Plant Breeding and Genetics Seminar Series, International Rice Research Institute, Los Banos, Philippines.

July 1989 “Screening and identification of American elm cell lines resistant to Dutch elm disease.” National Park Services of the Department of the Interior, Washington, DC. U.S.A.

Nov. 1989 “Cellular and molecular aspects to crop improvement” Dow /Elanco.Midland, MI.

PATENTS:

1. U.S. Patent #7,371,926. Continuation-In-Part: Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars for biofuel. May 13, 2008.

2. U.S. Patent #20050198704. Chloroplast transgenesis of monocots: Bioconfined genetically engineered monocot crops that will eliminate transgene flow.

2. U. S. Patent #7,049,485. Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars. Issues, June 1, 2006.

3. U.S. Patent Number: 6,100,456. Lepidopteran insect resistant transgenic potato plants. Issued August 2000...

4. U.S. Patent Number 5,767,368. Method for producing a cereal plant with foreign DNA. Issued June 16, 1998.

5. U.S. Patent Number 5,728,382. A supplement to item #3: A chitinase cDNA from a disease resistant American elm tree. Issued March 17, 1998.

6. U.S. Patent Number 5,539,095. A chitinase cDNA clone from a disease resistant Amer

7. U.S. Patent Number 5,281,529. Method for in vitro sexual reproduction of corn. Issued January 1994.

8. U.S. Patent Number 5,320,961. Method for in vitro asexual reproduction of corn. Issued June 1994.

PATENTS PENDING

9. U.S. Patent in progress: MSU 4.1-409, ID 98-004, Filed on May 26, 1998. Development of PHB, a biodegradable plastic in corn stocks. Filed in 1998

10. MSU 4.1-804A. Production of beta glucosidase, hemicellulase and ligninase in E1 and FLC-transgenic plants Patent application filed in 2007 Inventor: Mariam B. Sticklen.. MSU ID# 06068.

11. Field on Nov 20, 2007 as ID 99-047 Continuation of MSU 4.1-814 which is Continuation of MSU 4.1-539: Transgenic plants containing ligninase and cellulase that converts crop biomass to fermentable sugars.

12. Field as ID 08-013, MSU 4.1-877: Cont-In-Part of 4.1-814. Transgenic cover crops containing hemicellulase and cellulase which degrade hemicellulose and cellulose into fermentable sugars.

13. ID99-047; MSU 401-539; Serial #: 11/354,310; Cont-In-Part for Transgenic plants containing ligninase and cellulase that degrade lignin and cellulose for fermentable sugars.

14. ID 99-047; MSU 4.1-59; Serial #: 11/353,390. Cont-In-Part for Transgenic plants containing ligninase and cellulase that degrade lignin and cellulose for fermentable sugars.

15. Field in 2006. Inventor: Mariam B. Sticklen. Continuation in Parts. Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars. MSU 13172; ID# 4.1-539.

16. Full Patent application filed on Dec 1, 2007, and patent in progress. Inventor: Mariam B. Sticklen. Altering regulation of maize lignin biosynthetic enzymes via RNAi technology. . MSU-13579.

2007 Press Releases around the Globe on “Spartan Corn” as an Industrial Biotechnology Crop:

Cover page: CSA News (Crops, Soil, Agronomy) of the American Society of Agronomy/Crop Science Society of America/Soil Science Society of America. October 2007, Vol52, N10.

Press release displayed at the MSU Homepage in May, 2007.

Press release announced at the BIO 2007 in Boston, May 4, 2007

Biomass Magazine: Spartans Tailor Corn for Cellulosic ethanol. Page 11, July 2007.

MSU Alumni Magazine, page 22

MSU State News. Growing a new economy for Michigan: Biobased Technologies, page 17 & page 24

Daily Bioland and Biofuel Magazine on “Spartan Corn” by Harvey Forsat

Livestock Producers Magazine on Spartan Corn by Nadu Marley

NPR Radio interview

MSU News Bulletin, May 17, 2007

Investors Business Daily, May 12, 2007. Spartan Corn

Land Views Magazine in Florida, Nov 2007

Corn Genetic Engineering from Iowa, Adam Bossard

TV Interview by Detroit Radio/TV channel/Spped Channel on Biofuel from Cellulosic Biomass Feedstock and Logistics for Ethanol Production.

Amazon.com: Corn leaves and stalks to fuel cars. May, 7, 2007

Innovations Report: Germany: Creating corn for cars, May 7, 2007

huliq.com; HOLIC, NC-May 7, 2007: creating corn for cars.

English people.com.cn. People’s Daily Online, China-May 6, 2007: US Scientist Develop “ethanol Corn.”

English.people.com.cn, May 5, 07, People Daily Online, US Scientists develop “ethanol corn.” China

hindu.com/thehindu/holnus; Hindu, India-May 6, 2007: Creating Corn for Cars.

The Money Times, India-May 5, 2007: New Corn Variety Turns Stalks into Ethanol.

Earthtimes.org-May 4, 2007: New Corn Variety Turns Stalks into Ethanol.

imedianews.ge/en/news read; ImediNews, Georgia-May 4, 2007: New Corn Variety Turns Stalks into Ethanol.

SlipkaFinancial.com, May 2007, Corn Future to Go Big.

MapleWoodsHeating.com, May 2007, Reduce Your Heating Bill.

investmentu.com/Ethanol_Rpt, New Ethanol Report

tkfutures.com, May 2007, Corn Futures-Corn Options.

huliq.com/20930/Huliq: Breaking News, Creating Corn for Cars.

Earthtimes.org, Science and Technology Category, New corn variety turns stalks into ethanol, May 5, 2007.

LiveScience.com, May 7, 2008, Corn whose leaves and stalks carry an enzyme that breaks down cellulose for ethanol production.

sciencedaily.com/upi/index.php?feed=science. Science Daily. New corn variety turns stalks into ethanol.

www1.eere.energy.gov/biomass/news.html. Biomass News. With the Spartan Corn.

newsbulletin.msu.edu. may1707/bio2007.html. MSU News Bulletin. Two generations of Spartan Corn

www.gate2biotech.com. Spartan Corn.

www.isa.org/.../ContentManagement/ContentDisplay.cfm&ContentID=61618. ISA\Corn Plant Could Fuel Cheaper Ethanol Production.

www.theengineer.co.uk/liChannelID/128/Articles/299682/Ethanol+stalks.html: The Engineer News-[Production Engineering, maintenance…

www.blogtoplist.com/politics/blogdetails-610.html. Blog details Republican National Convention Blog-Blog Toplist

www.isaaa.org/rss/biofuels/default.asp. biofuel Supplement. Spartan Corn.

www.biotechknowledge.com/BIOTECH/knowcenter.nsf/ID/57BCC08124EFAD986257EA8?OpenDocument. Crop Biotech Update 11 May-2007 International Service: Spartan Corn for Cars

www.cedab.it/cgi-bin/newsletter/cbu-2007-05-11.pdf. ISAAA.org/KC-CropBiotech Update (11 May 2007)

CEDAB Cenro Documentazione Agrobiotecnologie. www.cedab.it/newsletter_ISAAA.asp?\Dnews=178. Spartan Corn

Scientific Blogging-Biology. www.scientificblogging.com/biology/feed: Spartan Corn

Plant Breeding and Genetics: Harvesting the Power of DNA. www.maes.msu.edu/publications/futures/spring2005/futures_spring 2005Golden Spartan

Zelfvenietigende mais-Chemisch 2 Weekblad. www.c2w.ni/4489_Zelfvernietigende_mais_.lynkx: Spartan Corn

Emerging Current: www.energycurrent.corn/index.php?id-3&storyid=2388: Spartan Corn. Turning corn wastes into ethanol feedstocks.

IndiaBlitz.com. www.indiablitz.corn/147971/: New variety of corn makes ethanol production for ….

What Michigan needs to compete. www.msu.edu/vpga/urc.swf. The Spartan Advantage Encourage lifelong learning ……

Experts’ database Office of Biobased Technology, Michigan State University. Bioeconomy.msu.edu/experts.aspx: Spartan Corn…

Ethanol Producer Magazine. Spartan corn germplasm has been genetically engineered to express cellulase and hemicellulase in the plant's leaves and stover. www.ethanolproducer.com/article.jsp?article_id=3160

Office of Biobased Technology. Spartan corn has enzymes included. Mariam Sticklen Breaking down cellulose and hemicellulose into simple sugars that can be fermented into ethanol has been ...
www.bioeconomy.msu.edu/achievements/spartancorn.aspx

Bioenergy pact between Europe and Africa. May 7, 2007 ... "We've developed two generations of Spartan Corn," said Mariam Sticklen, MSU professor of crop and soil sciences. "Both corn varieties ...
biopact.com/2007/05/third-generation-biofuels-scientists.html

NewsDaily: Science -- New corn variety turns stalks into ethanol. Mariam Sticklen, Michigan State University professor of crop and soil, says two generations of Spartan Corn contain enzymes necessary to break down ...
www.newsdaily.com/Science/UPI-1-20070308-10022500-bc-us-ethanol.xml

Biofuels News (Green Portal). May 7, 2007 ... But with the Spartan corn it is possible to turn corn leaves and stalks into ... efficient production of ethanol," said Mariam Sticklen, ...
www.checkbiotech.org/green_News_Biofuels.aspx?infoId=14602

New variety of corn makes ethanol production for cars more cost ...But with the Spartan corn it is possible to turn corn leaves and stalks into ... of ethanol," said Mariam Sticklen, MSU professor of crop and soil sciences. ...
in.news.yahoo.com/070505/139/6fdhd.html

AgBioView Newsletter on Agricultural Biotechnology. Aug 3, 2007 ... Spartan-carrying corn stover would be pulped, and the liquid containing the enzymes would be extracted, says researcher Mariam Sticklen. ...
www.agbioworld.org/newsletter_wm/index.php?caseid=archive&newsid=2754

Cropbiotech Update 11 May 2007 11-May-2007 International Service ... May 11, 2007 ... SPARTAN CORN FOR CARS. The leaves and stalks of a new corn variety ... Mariam Sticklen, an MSU professor of crop and soil sciences, ...
www.biotechknowledge.com/BIOTECH/knowcenter.nsf/ID/57BCC081240EFAD9862572D800495EA8?OpenDocument

Plant Life. Mariam Sticklen developed a series of corn genetic lines that grow the enzymes ... The Spartan Corn series promises to make a waste product commercially ...
www.msu.edu/~rohler/f07report/may.htm

First American cellulosic ethanol plant now in production ... He criticizes corn ethanol (and more recently cellulosic ethanol) as requiring more fossil .... and Mariam Sticklen, professor of crop and soil sciences, ...
www.gminsidenews.com/forums/showthread.php?t=60232

GRASSOLINE. Mariam Sticklen, professor of crop and soil sciences, have found a way to make a corn ... on the first and second generations of this Spartan Corn, and ...
www.bioeconomy.msu.edu/news/dale_msualumnimag_winter08.pdf

SeedQuest. "We've developed two generations of Spartan Corn," said Mariam Sticklen, MSU professor of crop and soil sciences. "Both corn varieties contain the enzymes ...
www.seedquest.com/News/releases/2007/may/19171.htm

ISA | Corn plant could fuel cheaper ethanol production. “We’ve developed two generations of Spartan Corn,” said Mariam Sticklen, MSU professor of crop and soil sciences. “Both corn varieties contain the enzymes ...
www.isa.org/.../ContentManagement/ContentDisplay.cfm&ContentID=61618

Creating corn for cars : International. We've developed two generations of Spartan Corn, said Mariam Sticklen, MSU professor of crop and soil sciences. Both corn varieties contain the enzymes ...
www.nerve.in/news:25350047278

New variety of corn makes ethanol production for cars more cost ... But with the Spartan corn it is possible to turn corn leaves and stalks into ... efficient production of ethanol,â€ said Mariam Sticklen, MSU professor of ...
www.ebiologynews.com/1853.html

ZAMP Bionews » Blog Archive » New variety of corn makes ethanol ... But with the Spartan corn it is possible to turn corn leaves and stalks into ... of ethanol,” said Mariam Sticklen, MSU professor of crop and soil sciences. ...
www.zampbioworld.org/bionews/index.php/2007/05/05/77

AgBioView Newsletter on Agricultural Biotechnology. Michigan State University scientist Mariam Sticklen developed a genetically modified corn line called Spartan that produces its own natural cellulase enzyme ...
www.agbioworld.org/newsletter_wm/index.php?caseid=archive&newsid=2796

East North Central US Biotechnology & GMOs News - Agriculture ... Production Frontiers: Spartan Corn 6 Feb 2008 20:52 GMT ... EAST LANSING, Mich. ... Michigan State University scientist Mariam Sticklen developed a . ...
agriculture.einnews.com/news/east-north-central-us-biotechnology-gmos?offset=50

Ethanol stalks - The Engineer Online - [Production Engineering ... ‘We've developed two generations of Spartan Corn,’ said Mariam Sticklen, MSU professor of crop and soil sciences. ‘Both corn varieties contain the enzymes ...
secure.theengineer.co.uk/Articles/299682/Ethanol+stalks.htm

CEDAB Centro Documentazione Agrobiotecnologie. Spartan Corn for CarsThe leaves and stalks of a new corn variety developed by Michigan ... Mariam Sticklen, an MSU professor of crop and soil sciences, ...
www.cedab.it/newsletter_ISAAA.asp?IDnews=178

Plant Breeding and Genetics: Harvesting the Power of DNA. …said Mariam Sticklen, professor of crop and soil sciences, who has been. Filling Up ..... Spartan 162. 1958. Golden Spartan. 1974. Corn (Open-pollinated) ...
www.maes.msu.edu/publications/futures/spring2005/futures_spring2005.pdf

ISAAA.org/KC - CropBiotech Update (11 May 2007). SPARTAN CORN FOR CARS. The leaves and stalks of a new corn variety developed by Michigan ... Mariam Sticklen, an MSU professor of crop and soil sciences, ...
www.isaaa.org/Kc/cropbiotechupdate/2007/05/11.html

Wade Clisby Page 1 of 1 Untitled Page 24/05/2007. Mariam Sticklen, MSU Professor of Crop and Soil Sciences,. announced that she and her colleagues have developed two generations of Spartan Corn. ...
claria12.securesites.net/hosting/seedworld/seedweek/pdf/8May07.pdf

MSU Today. “…generations of Spartan Corn," said Mariam Sticklen, MSU professor of crop and soil sciences. "Both corn varieties contain the enzymes necessary . ...
www.msutoday.com/search/?page=1&perPage=10&search=crop&sort=title

ISAAA.org/KC - CropBiotech Update (11 May 2007). SPARTAN CORN FOR CARS. The leaves and stalks of a new corn variety developed ... Mariam Sticklen, an MSU professor of crop and soil sciences, said that not ...
www.cedab.it/cgi-bin/newsletter/cbu-2007-05-11.pdf

East North Central US Wheat News - Agriculture Industry Today. Production Frontiers: Spartan Corn 6 Feb 2008 20:52 GMT ... Michigan State University scientist Mariam Sticklen developed a genetically . ...
agriculture.einnews.com/news/east-north-central-us-wheat?offset=50

First American cellulosic ethanol plant now in production [Archive ... If more ethanol comes from sources such as this rather than corn, I think people will warm ..... and Mariam Sticklen, professor of crop and soil sciences, ...
www.gminsidenews.com/forums/archive/index.php?t-60232.html

はてなブックマーク - PoePoeのブックマーク / 環境 [ Translate this page ] We've developed two generations of Spartan Corn," said Mariam Sticklen, MSU professor of crop and soil sciences. "... First Successful Demonstration of ...
b.hatena.ne.jp/PoePoe/環境/?mode=detail

PUBLICATIONS:

a)Books

8. Kirk T., Carlson J., Ellstrand N., Kapuscinski, Lumpkin T., Magnus D., Nester E., Peloquin J, Snow A., Sticklen M., and Turner P. (2004). Biological Confinement of Genetically Engineered Organisms. NRC Report. The Natl. Acad. Press. Washington, DC. 255p.

9. Mariam B. Sticklen and James L. Sherald, eds. (1992). Dutch elm disease: Cellular and Molecular Approaches. Springer-Verlag Publs. New York, N.Y. 344 p.

10. M. B. Sticklen and M. Kenna (1998). Cellular and Molecular Genetics Approaches to Turfgrass Improvement. Ann Arbor Press. Ann Arbor, MI. 256 p.

b) Refereed Publications (In print and in press)

11. M. Sticklen (2008). Plant genetic engineering for biofuel production: Towards affordable cellulosic ethanol. Nature Review Genet. 9 (6): In press. (Invited Review).

12. M. Sticklen (2007). Feedstock genetic engineering for biofules. Crop Science. 47: 2238-2248 (Invited Review)

12. H. Oraby, B. Venkatesh, B. Dale, R. Ahmad, C. Ransom, J. Oehmke and M. Sticklen (2007). Enhanced conversion of plant biomass into glucose using transgenic rice-produced endoglucanase for cellulosic ethanol. Transgenic Res. 16(6): 739-749.

13. C. Ransom, Venkatesh B., Dale B., Biswas G. and Mariam Sticklen (2007). Heterologous Acidothermus cellulolyticus 1,4-β-endoglucanase E1 Produced within the Corn Biomass Converts Corn Stover into Glucose Applied Biochem. Biotech. 140: 137-219.

14. M. Sticklen (2006). Plant genetic engineering to improve biomass characterization for biofuels. Curr. Opin. Biotechnology. 17(3): 315-319. (Invited review).

15. G. Biswas, Ransom C., and Mariam Sticklen (2006). Expression of biologically active Acidothermus cellulolyticus endoglucanase in transgenic maize. Plant Sci. 171: 617-623.

16. Najafi F., Rastgar F., Khavari-Nejad, R. A., and M. B. Sticklen (2005). Salt tolerance in transgenic pea (Pisum sativum L.) plants by P5CS gene transfer. J. Plant Biotechnology. 7(4): 1-8.

17. Hesham F. Oraby, Callista B. Ransom, Alexandra N. Kravchenko and Mariam B. Sticklen (2005). Barley HVA1 Gene Confers Salt Tolerance in R3 Transgenic Oat. Crop Science. 45(6): 2 2218-2227.

18. Sticklen M. B. and H. Oraby (2005). Shoot apical meristem: A sustainable explant for genetic engineering of cereal crops. In Vitro Cellular & Developmental-PLANT. 41: 187-200

19. Salehi, H., Z. Seddighi, A.N. Kravchenko and M.B. Sticklen (2005). Expression of the cry1Ac in common bermudagrass (Cynodon dactylon [L.] Pers. ‘Arizona Common’) via Agrobacterium-mediated transformation and control of black cutworm (Agrostis ipsilon Hufnagel). J. Amer. Soc. Hort. Sci. 130:619-623.

20. Salehi H., Ransom C., Oraby H., and Sticklen M. (2005). Delay in flowering and increase in biomass of plants expressing the Arabidopsis floral repressor gene FLC (FLOWERING LOCUS C). J. Plant Physiology. 162: 711-717.

21. Ahmad, A., Maqbool, S.B., Hashsham, S.A., Sticklen, M.B. (2005). Determination of cryIAb and cryIAc copy number in transgenic Basmati 370 Rice (Oryza sativa L.) plants using Real-time PCR and its comparison with Southern Blot. Journal of Biological Sciences 5:283-288.

22. Teymouri F., Alizadeh H., Laureano-Preze L., Dale B., and Sticklen M. (2004). Effects of Ammonia fiber explosion (AFEX) on the activity of heterologous cellulase enzyme of transgenic plants. Applied Biochemistry & Biotechnology. 16: 1183-1191

23. Maqbool S. and M. B. Sticklen (2004). Genetically engineered turfgrasses for pest resistance. USGA Turfgrass and Environmental Research. 2(2): 1-13. 2004.

24. Ahmad A., S. Maqbool, S. Hashshsham, and M. B. Sticklen (2005). Determining of cry1Ac copy number in transgenic Basmati 370 rice (Oryza sativa L.) plants and its comparison with Southern blot. J. Biological Sciences. 5: 283-288.

25. Salehi H., Ransom C., Oraby H., and Sticklen M. (2005). Delay in flowering and increase in biomass of plants expressing the Arabidopsis floral repressor gene FLC (FLOWERING LOCUS C). J. Plant Physiology. In press.

26. H. Zhong, F. Teymouri, B. Chapman, S. Maqbool, R. Sabzikar, Y. El-Maghraby, B. Dale, and M. B. Sticklen. (2003). the dicot pea (Pisum sativum L.) rbcS transit peptide directs the Alcaligenes eutrophus polyhydroxybutyrate enzymes into the monocot maize (Zea mays L.) chloroplasts. Plant Sci. 165: 455-462.

27. Prathibha Devi and Mariam Sticklen (2003). In vitro culture and genetic transformation of sorghum by microprojectile bombardment. Plant Biosystems (Italy) 137: (3) 249-254.

28. A. Ahmad, H. Zhong, W. Wang and M. B. Sticklen. (2002). Shoot apical meristem: In vitro regeneration and morphogenesis in wheat (Triticum aestivum L.). In-Vitro Cellular & Developmental Biology (IVCDB)-PLANT. 38: 163-167.

29. P. Devi and M. B. Sticklen (2002). Genetic engineering of pearl millet though meristem primordia system. Euphytica. 125: 145-150

30. P. Devi & M. B. Sticklen (2001) Culturing shoot-tip clumps of pearl millet [Pennisetum glaucum (L.) R. Br.] and optimal microprojectile bombardment parameters for transient expression. Euphytica 125: 45-50.

31. P. Devi & M. B. Sticklen (2001) Culturing shoot-tip clumps of Sorghum bicolor (L.) Moench and optimal microprojectile bombardment parameters for transient expression. J. Cytol. Genet. 2 (NS): 89-96.

32. S. Maqbool, P. Devi and Mariam Sticklen. (2001). Biotechnology: Advances for the Genetic improvement of sorghum (Sorghum bicolor (L.) Moench). Special Issue, In vitro Cell. Dev. Biol. Plant. 37: 5.

33. P. Devi, H. Zhong, M.B. Sticklen. (2000). In vitro morphogenesis of pearl millet [Pennisetum glaucum (L.) R. Br.]: Efficient production of multiple shoots and inflorescences from shoot apices. Plant Cell Rep. 19 (6): 546-550.

34. S. Maqbool, H. Zhong, Y. El-Maghraby, W. Wang, A. Ahmad, B. Chai and M. B. Sticklen (2002). Competence of oat (Avena sativa L.) shoot apical meristems for integrative transformation, inherited expression and osmatic tolerance of hva1 transgene. Theor. Appli. Genet. 105:201-208.

35. C.-A. Liu, H. Zhong, J. Vargas, D. Penner, and M. B. Sticklen (1998). Prevention of fungal diseases in transgenic bialaphos and glufosinate-resistant creeping bentgrass (Agrostis palustris). Weed Science 46: 139-146.

36. H. Zhong, W. Wang and M. B. Sticklen (1998). In vitro morphogenesis of Sorghum bicolor (L.) Moench: Efficient plant regeneration from shoot apices. J. Plant Physiol. 153: 719-726.

37. M. B. Sticklen and M. G. Bolyard (1993). Cerato-ulmin: a hydrophobic fungal toxin associated with Dutch elm disease. Trends in Microbiology. 2: 213-217.

38. B. Chai and M. B. Sticklen (1998). Application of biotechnology in turfgrass improvement. A review paper in Crop Sci.38: 1320-1338.