H. T. Tien and Angelica Ottova (Editors)
Contents
Chapter 1: The lipid bilayer concept: Experimental
realization and current applications
H. T. Tien and Angelica Ottova
Chapter 2: Dielectric and Electrical Properties
of Lipid Bilayers in Relation to their Structure
Hans G. L. Coster
Chapter 3: Boundary potentials of bilayer lipid
membranes: methods and interpretations
Yu. A. Ermakov and V. S. Sokolov
Chapter 4: Effect of anisotropic properties of
membrane constituents on of membrane bilayer structures
Ales Iglic and Veronika Kralj-Iglic
Chapter 5: Elastic properties of BLMs and pore
formation
Dumitru Popescu, Stelian Ion,
Aurel Popescu, and Liviu Movileanu
Chapter 6: Mechanoelectric properties of BLMs
Alexander G. Petrov
Chapter 7: Chain-ordering
phase transition in bilayer: Kinetic mechanism and its physiological implications
Dmitri Kharakoz
Chapter 8: Coupling
of chain melting and bilayer structure
Thomas Heimburg
Chapter 9: Water transport across membranes
Peter Pohl
Chapter 10: Biopolymer induced structural modifications
of lipid bilayers
Sylvio May and A. Ben-Shaul
Chapter 11: Investigation of substrate-specific
porin channels in BLMs
Roland Benz
Chapter 12: Planar lipid bilayer analyses of bacterial
porins: the role of structure in defining function
M. A. Arbing and J. W. Coulton
Chapter 13: Reconstitution in planar lipid bilayers
of ion channels synthesized in vivo and in vitro
L. K. Lyford and R. L. Rosenberg
Chapter 14: Multi-channel and single-channel investigation
of protein and peptide incorporation into BLMs
E. Gallucci, S. Micelli, and
V. Picciarelli
Chapter 15: Structure and function of plant membrane
ion channels reconstituted in planar lipid bilayers
M. Smeyers, M. Léonetti,
E. Goormaghtigh, and F. Homblé
Chapter 16: Reconstituting SNARE proteins into
BLMs
K. T. Rognlien and D. J. Woodbury
Chapter 17: The mitochondrial ion channels in
planar lipid bilayer and in native mitochondria
Galina Mironova
Chapter 18: The use of liposomes to detect channel
formation mediated by secreted bacterial proteins
V. Cabiaux, S. Vande Weyer,
and J.M. Ruysschaert
Chapter 19: Memory effect on ion channels by using
BLM
R. Cassia-Moura
Chapter 20: Symmetric and asymmetric planar lipid
bilayers of various lipid composition
A. Wiese, T. Gutsmann, and U.
Seydel
Chapter 21: Insights into ion channels from peptides
in planar lipid bilayers
Herve Duclohier
Chapter 22: Permeation property and intramembrane
environments of synthetic phytanyl-chained glycolipid membranes
T. Baba, H. Minamikawa, M. Hato,
and T. Handa
Chapter 23: Modulation of planar bilayer permeability
by electric fields and exogenous peptides
A. Gliozzi, F. Gambale, and
G. Menestrina
Chapter 24: Gravitational impact on ion channels
incorporated into planar lipid bilayers
M. Wiedemann, H. Rahmann, and
W. Hanke
Chapter 25: Advantages and disadvantages of patch-clamping
M. L. Kelly and Dixon J. Woodbury
Chapter 26: Pharmacology of intracellular calcium
channels
Peter Koulen
Chapter 27: Systems aspects of supported membrane
biosensors
I. R. Peterson and J. A. Beddow
Chapter 28: Biosensors from interactions of DNA
with lipid membranes
U. J. Krul, D. P. Nikolelis,
and J. Zeng
Chapter 29: Structure and electrochemistry of
fullerene-lipid hybrid and composite materials
Naotoshi Nakashima
Chapter 30: Analytical applications of bilayer
lipid membrane systems
Marek Trojanowicz
Chapter 31: Transmembrane voltage sensor
J. A. Cohen, B. Gabriel, J.
Teissié, M. Winterhalter
Chapter 32: Domains, cushioning, and patterning
of bilayers by surface interactions with solid substrates and their sensing
properties
Ruxandra Vidu, Timothy V. Ratto,
Marjorie L. Longo, and Pieter Stroeve
Chapter 33: Supported BLMs as biosensors
Angelica Ottova, Vladimir Tvarozek
and HT Tien
Chapter 34: Photoinduced Charge Separation in
Lipid Bilayers
D. Mauzerall and K. Sun
Chapter 35: Photosynthetic pigment-protein complexes
in planar lipid membranes
W. I. Gruszecki and A. Wardak
Chapter 36: Biochemical Applications of Solid
Supported Membranes on Gold Surfaces
Andreas Janshoff, Hans-Joachim
Galla, and Claudia Steinem
Chapter 37: Simultaneous measurement of spectroscopic
and physiological signals from a planar bilayer system
Yoshiro Hanyu
Subject Index
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Planar Lipid Bilayers (BLMs)
and their Applications
Edited by HT Tien and A. Ottova
(Elsevier Science, Amsterdam, New York, 2003)
This book is about the most basic structural element of cell membranes, namely, the lipid bilayer, without which the life as we know it is not possible. Thus, in this volume, comprising 37 contributed chapters, researchers who have devoted decades of their scientific career on the lipid bilayer, have written many of them. The topics covered stretch from the origins of the lipid bilayer concept and its experimental realization to membrane biophysics, ion channels reconstitution, a variety of lipid bilayer-based biosensors and molecular devices, to light-induced charge separation and experimental techniques. Specifically, the book consists of 4 major sections:
In one authoritative volume, this book should appeal to those interested in the applications of self-assembled lipid bilayers on polymers, metallic supports, and microchips. This is because of recent advances in microelectronics coupled with membrane research of the past decades have come of age and are poised for biotechnological exploitation.
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As stated by the Editors in the Preface:
“… about four decades ago at a symposium on the Plasma Membrane, organized jointly by the American and New York Heart Association, Donald Rudin and his associates reported in a paper entitled ‘Reconstitution of Cell Membrane Structure in vitro and its Transformation into an Excitable System’ (Nature, 194, 979, 1962). As evidenced by the present volume, the rest as they say was history. The reconstituted system has been known under various names (black, bimolecular, bilayer lipid membrane, BLM for short, or simply planar lipid bilayer). Call whatever name you prefer, a conventional BLM about 5 nm thick is interposed between two aqueous solutions. It is, together with lipid vesicles (liposomes), the most widely used experimental model of biomembranes. This liquid-crystalline BLM, embodied in the lipid bilayer principle of biomembranes, and …. beyond performing as a physical boundary, has evolved, to serve:
• as a discriminatory barrier
• as a conduit for transport
• as a reactor for energy conversion
• as a transducer for signal
processing
• as a bipolar electrode for
redox reactions
• as a site for molecular recognition
and/or
• other diverse purposes such
as apoptosis, AIDS and Alzheimer’s disease research, etc.“
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Today, from all lines of experimental findings, there is little doubt that all biomembranes possess a lipid bilayer structure, thereby underlying the lipid bilayer principle of biomembranes. The lipid bilayer research has evolved as an interdisciplinary effort, benefited by a cross-fertilization of ideas. The lipid bilayer associated with basic sciences and biotechnology is of fundamental interest to a wide diversity of investigators.*
*Biochemists, Biologists, Biophysicists
Bioengineers and technologists
Electrochemists, Physiologists, Pharmacologists
Surface and colloid scientists, and
Others working on ultrathin films and membrane
phenomena.
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To mark the 40th anniversary of the discovery of the BLM, an article has been prepared for the occasion The Lipid Bilayer Concept and Its Experimental Realization: From Soap Bubbles, the Kitchen Sink to Black Lipid Membranes, J. Memb. Sci., 189, 2001, 83-117.
If interested, email to: ottova@msu.edu
Further interesting links: http://www.msu.edu/user/ottova/membrane.biophysics.html
http://www.msu.edu/user/ottova/soap_bubble.html
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