MEMBRANE  BIOPHYSICS
 AS VIEWED FROM EXPERIMENTAL BILAYER
LIPID MEMBRANES
(Planar Lipid Bilayers and Spherical Liposomes)

Published by Elsevier Science, Amsterdam and New York, 2000, 648 pp.



Chapter 7

Membrane Physiology

“…action potential (AP) is a result of the movement of ions…., leading to electrical signaling in a language …the brain understands. AP is a propagating wave of electrical depolarization… driven by the transmembrane electrochemical potential gradients of Na+ and K+. … maintained by molecular pumps, which derive their energy from the hydrolysis of ATP.”

7.1    Introduction7.2    The Nerve Membrane
            Resting and Action Potentials
            Cyto-organelle Membrane and the Gap Junction
            Muscle Membranes
7.3    Sensory Transduction
7.4    Measurement Techniques
            Voltage Clamp
            Patch-clamp
7.5    Ion Channels and Their Reconstitution in Planar BLMs and  Liposomes
            K+ channels, Na+ channels, Ca2+ channels
            Anion channels and other channels
7.6    Other BLM and Liposome Experiments
 

General References

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     Gaegurin 4, a peptide antibiotic of frog skin, forms voltage-dependent channels in planar lipid bilayers

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     Functional dissection of the R domain of cystic fibrosis transmembrane conductance regulator

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     Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci
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     Complement activation by bacterial surface glycolipids: A study with planar bilayer membranes

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     Inhibition of cardiac L-type calcium channels by epoxyeicosatrienoic acids

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     Heparin influence on alpha-staphylotoxin formed channel

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     Genetically engineered metal ion binding sites on the outside of a channel's transmembrane beta-barrel

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     Recombinant pI(Cln) forms highly cation-selective channels when reconstituted into artificial and
biological membranes

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     Simultaneous measurement of spectroscopic and physiological signals from a planar bilayer system:
Detecting voltage-dependent movement of a membrane-incorporated peptide

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     Artificial ion channels of amphiphilic ion pairs composed of oligoether-ammonium and hydrophobic
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     Membrane trafficking of the cystic fibrosis gene product, cystic fibrosis transmembrane conductance
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     Single-channel properties of inositol (1,4,5)-trisphosphate receptor heterologously expressed in HEK-
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    Yoshihara E, Yoneyama H, Ono T, et al. BIOCHEM BIOPH RES CO 247: (1) 142-145 JUN 9 1998
     Identification of the catalytic triad of the protein D2 protease in Pseudomonas aeruginosa

    Lupu VD, Kaznacheyeva E, Krishna UM, et al. J BIOL CHEM 273: (23) 14067-14070 JUN 5 1998
     Functional coupling of phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate receptor

    Zahradnikova A, Meszaros LG J PHYSIOL-LONDON 509: (1) 29-38 MAY 15 1998
     Voltage change-induced gating transitions of the rabbit skeletal muscle Ca2+ release channel

    Kaulin YA, Schagina LV, Bezrukov SM, et al. BIOPHYS J 74: (6) 2918-2925 JUN 1998
     Cluster organization of ion channels formed by the antibiotic syringomycin E in bilayer lipid
membranes

    Rostovtseva TK, Bezrukov SM BIOPHYS J 74: (5) 2365-2373 MAY 1998
      ATP transport through a single mitochondrial channel, VDAC, studied by current fluctuation analysis

Shafir I, Feng W, Shoshan-Barmatz V EUR J BIOCHEM 253: (3) 627-636 MAY 1 1998
Dicyclohexylcarbodiimide interaction with the voltage-dependent anion channel from sarcoplasmic reticulum

    Edwards JC, Tulk B, Schlesinger PH J MEMBRANE BIOL 163: (2) 119-127 MAY 15 1998
     Functional expression of p64, an intracellular chloride channel protein

    Ruysschaert JM, Goormaghtigh E, Homble F, et al. FEBS LETT 425: (2) 341-344 MAR 27 1998
     Lipid membrane binding of NK-lysin

    Tripathy A, Tien HT, Ottova AL BIOELECTROCH BIOENER 44: (2) 183-199 JAN 1998
     A study of reconstituted anion channels using lipid bilayers

    Wiese A, Munstermann M, Gutsmann T, et al. J MEMBRANE BIOL 162: (2) 127-138 MAR 15 1998
     Molecular mechanisms of polymyxin B Membrane interactions: Direct correlation between surface
charge density  and self-promoted transport

    Radke PW, Frenkel C, Urban BW EUR J ANAESTH 15: (1) 89-95 JAN 1998
     Molecular actions of droperidol on human CNS ion channels

    Clancy JP, Bebok Z, Sorscher EJ J BIOENERG BIOMEMBR 29: (5) 475-482 OCT 1997
     Purification, characterization, and expression of CFTR nucleotide-binding domains

    Zahradnikova A, Minarovic I, Venema RC, et al. CELL CALCIUM 22: (6) 447-453 DEC 1997
     Inactivation of the cardiac ryanodine receptor calcium release channel by nitric oxide

    Tien HT, Barish RH, Gu LQ, et al. ANAL SCI 14: (1) 3-18 FEB 1998
     Supported bilayer lipid membranes as ion and molecular probes

    Gokel GW, Abel E, De Wall SL, et al. AN QUIM-INT 93: (5) 347-354 1997
     Synthetic organic models for transmembrane, cation conducting channels

   Reumann S, Maier E, Heldt HW, et al. EUR J BIOCHEM 251: (1-2) 359-366 JAN 15 1998
     Permeability properties of the porin of spinach leaf peroxisomes

    Shevchenko S, Feng W, Varsanyi M, et al. J MEMBRANE BIOL 161: (1) 33-43 JAN 1 1998
     Identification, characterization and partial purification of a thiol-protease which cleaves specifically the
skeletal muscle ryanodine receptor Ca2+ release channel

    Luo YG, Glisson JR, Jackwood MW, et al. J BACTERIOL 179: (24) 7856-7864 DEC 1997
     Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73

    Helluin O, Dugast JY, Molle G, et al. BBA-BIOMEMBRANES 1330: (2) 284-292 DEC 4 1997
     Lateral diffusion and conductance properties of a fluorescein-labelled alamethicin in planar lipid bilayers

    Bezrukov SM, Vodyanoy I BIOPHYS J 73: (5) 2456-2464 NOV 1997
     Signal transduction across alamethicin ion channels in the presence of noise

    Salamon Z, Macleod HA, Tollin G BIOPHYS J 73: (5) 2791-2797 NOV 1997
     Coupled plasmon-waveguide resonators: A new spectroscopic tool for probing proteolipid film structure and properties

    Krasilnikov OV, Yuldasheva LN, Merzlyak PG, et al. BBA-BIOMEMBRANES 1329: (1) 51-60 1997
     The hinge portion of the S-aureus alpha-toxin crosses the lipid bilayer and is part of the trans-mouth of the channel

    Radecki J, Radecka H SENSOR ACTUAT B-CHEM 42: (2) 145-148 JUL 31 1997
     Potentiometric determination of selective interaction of odorants on black lipid membranes

    Shin JH, Chung S, Park EJ, et al. FEBS LETT 415: (3) 299-302 OCT 6 1997
     Nitric oxide directly activates calcium-activated potassium channels from rat brain reconstituted into planar lipid bilayer

    Carneiro CMM, Krasilnikov OV, Yuldasheva LN, et al. FEBS LETT 416: (2) 187-189 OCT 20 1997
     Is the mammalian porin channel, VDAC, a perfect cylinder in the high conductance state?

    Yoshida K, Ide T, Inouye K, et al. BBA-BIOMEMBRANES 1325: (2) 178-188 APR 26 1997
     A voltage- and K+-dependent K+ channel from a membrane fraction enriched in contractile vacuole of Dictyostelium discoideum

    Salamon Z, Macleod HA, Tollin G BBA-REV BIOMEMBRANES 1331: (2) 117-129 SEP 8 1997
     Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein systems .1. Theoretical principles

    Salamon Z, Macleod HA, Tollin G
     Surface plasmon resonance spectro BBA-REV BIOMEMBRANES 1331: (2) 131-152 SEP 8 1997
scopy as a tool for investigating the biochemical and biophysical properties of
     membrane protein systems .2. Applications to biological systems

    Schwartz JL, Potvin L, Chen XJ, et al. APPL ENVIRON MICROB 63: (10) 3978-3984 OCT 1997
     Single-site mutations in the conserved alternating-arginine region affect ionic channels formed by CryIAa, a Bacillus thuringiensis toxin

    Guihard G, Rousseau E., M S-MED SCI 13: (10) 1157-1164 OCT 1997
     Are ion channels of the nuclear envelope involved in nuclear function?

    Picher M, Decrouy A, Proteau S, et al. BBA-BIOMEMBRANES 1328: (2) 243-260 SEP 4 1997
     Conducting and voltage-dependent behaviors of the native and purified SR Ca2+-release channels from the canine diaphragm

    Huynh PD, Cui C, Zhan HJ, et al. J GEN PHYSIOL 110: (3) 229-242 SEP 1997
     Probing the structure of the diphtheria toxin channel - Reactivity in planar lipid bilayer membranes of
     cysteine-substituted mutant channels with methanethiosulfonate derivatives

    Eliassi A, Garneau L, Roy G, et al. J MEMBRANE BIOL 159: (3) 219-229 OCT 1 1997
     Characterization of a chloride-selective channel from rough endoplasmic reticulum membranes of rat hepatocytes: Evidence for a block by phosphate

    Voyer N, Potvin L, Rousseau E.,  J CHEM SOC PERK T 2 (8) 1469-1471 AUG 1997
     Electrical activity of artificial ion channels incorporated into planar lipid bilayers

    Feng W, ShoshanBarmatz V EUR J BIOCHEM 247: (3) 955-962 AUG 1 1997
     Involvement of lysine residues in the gating of the ryanodine receptor Ca2+-release channel of skeletal muscle  sarcoplasmic reticulum

Das S, Lengweiler UD, Seebach D, et al. P NATL ACAD SCI USA 94: (17) 9075-9079 AUG 19 1997
     Proof for a nonproteinaceous calcium-selective channel in Escherichia coli by total synthesis from
     (R)-3-hydroxybutanoic acid and inorganic polyphosphate

    Bhat MB, Zhao JY, Takeshima H, et al. BIOPHYS J 73: (3) 1329-1336 SEP 1997
     Functional calcium release channel formed by the carboxyl-terminal portion of ryanodine receptor

    CassiaMoura R, Xavier IM BIOELECTROCH BIOENER 42: (2) 193-195 MAY 1997
     Is it possible to induce ion channel memory control?

    Gruszecki WI, Wardak A, Maksymiec W.,  J PHOTOCH PHOTOBIO B 39: (3) 265-268 JUL 1997
     The effect of blue light on electron transport in photosystem II reconstituted in planar bilayer lipid membrane

    Schwartz JL, Lu YJ, Sohnlein P, et al. FEBS LETT 412: (2) 270-276 JUL 28 1997
     Ion channels formed in planar lipid bilayers by Bacillus thuringiensis toxins in the presence of Manduca sexta midgut receptors

    Grosman C, Reisin IL.,  J MEMBRANE BIOL 158: (1) 87-94 JUL 1 1997
     Interconverting gating modes of a nonselective cation channel from the tapeworm Echinococcus granulosus reconstituted on planar lipid bilayers

    Schwartz JL, Juteau M, Grochulski P, et al. FEBS LETT 410: (2-3) 397-402 JUN 30 1997
     Restriction of intramolecular movements within the Cry1Aa toxin molecule of Bacillus thuringiensis through disulfide bond engineering

    Krasilnikov OV, Merzlyak PG, Yuldasheva LN, et al. MED MICROBIOL IMMUN 186: (1) 53-61 1997
     Pore-forming properties of proteolytically nicked staphylococcal alpha-toxin: the ion channel in planar lipid bilayermembranes

    Kaile A.,  J EXP BOT 48: (309) 979-983 APR 1997
     Cholesterol-modulated ion channel activity in tonoplast vesicles: A planar lipid bilayer study

    White PJ.,  J EXP BOT 48: 499-514 Sp. Iss. SI MAR 1997
     Cation channels in the plasma membrane of rye roots

Ikigai H, Ono T, Iwata M, et al. FEMS MICROBIOL LETT 150: (2) 249-254 MAY 15 1997
     El Tor hemolysin of Vibrio cholerae O1 forms channels in planar lipid bilayer membranes

    Ismailov II, Berdiev BK, Shlyonsky VG, et al. AM J PHYSIOL-CELL PH 41: (4) C1077-C1086 1997
     Role of actin in regulation of epithelial sodium channels by CFTR

    Hirsh JK, Wu CH TOXICON 35: (2) 169-176 FEB 1997
     Palytoxin-induced single-channel currents from the sodium pump synthesized by in vitro expression

    Welch W, Williams AJ, Tinker A, et al. BIOCHEMISTRY-US 36: (10) 2939-2950 MAR 11 1997

     Structural components of ryanodine responsible for modulation of sarcoplasmic reticulum calcium channel function

    Krasilnikov OV, Capistrano MFP, Yuldasheva LN, et al. J MEMBRANE BIOL 156: (2) 157-172 MAR 15 1997

     Influence of Cys-130 S-aureus alpha-toxin on planar lipid bilayer and erythrocyte membranes

    Ma XH, Shi YL BIOCHEM BIOPH RES CO 232: (2) 461-463 MAR 17 1997
     Effects of ADP, DTT, and Mg2+ on the ion-conductive property of chloroplast H+-ATPase(CF0-CF1) reconstituted into bilayer membrane

    Pohl P, Saparov SM, Antonenko YN BIOPHYS J 72: (4) 1711-1718 APR 1997
     The effect of a transmembrane osmotic flux on the ion concentration distribution in the immediate membrane vicinity measured by microelectrodes

    Juteau M, Prefontaine G, Grochulski P, et al. BIOPHYS J 72: (2) MP302-MP302 Part 2 FEB 1997
  Planar lipid bilayer studies of disulphide bridge engineered Cry1Aa, a Bacillus thuringiensis insecticidal toxin.

    Racape J, Granger D, Noulin JF, et al. BIOPHYS J 72: (2) MP329-MP329 Part 2 FEB 1997
     Properties of the pores formed by parental and chimeric Bacillus thuringiensis insecticidal toxins in planar lipid     bilayer membranes.

    Chung SK, Shin JH, Suh CK, et al. BIOPHYS J 72: (2) WPO87-WPO87 Part 2 FEB 1997
     Nitric oxide (NO) directly activates Ca2+-activated K+ channels from rat brain reconstituted into planar
lipid bilayer.
Shin JH, Park EJ, Suh CK BIOPHYS J 72: (2) TH302-TH302 Part 2 FEB 1997
     Channel-like activities of an antimicrobial peptode, buforin II, observed in planar lipid bilayer

    Yamada T, Hanyu Y, Matsumoto G BIOPHYS J 72: (2) TH303-TH303 Part 2 FEB 1997
     Fluorescence measurement of peptide channel gating in planar lipid bilayer.

    Feigin AM, Schagina LV, Takemoto JY, et al. BBA-BIOMEMBRANES 1324: (1) 102-110 FEB 21 1997
     The effect of sterols on the sensitivity of membranes to the channel-forming antifungal antibiotic, syringomycin E

    Schatz A, LinkeHommes A, Neubert J EUR BIOPHYS J BIOPHY 25: (1) 37-41 1996
     Gravity dependency of the gramicidin A channel conductivity - A model for gravity perception on the cellular level

    Alix SN, Woodbury DJ BIOPHYS J 72: (1) 247-253 JAN 1997
     Phospholipase A(2) action on planar lipid bilayers generates a small, transitory current that is voltage independent

    Chan HC, Zhou TS, Fu WO, et al. BBA-BIOMEMBRANES 1323: (1) 117-129 JAN 14 1997
     Cation and anion channels in fat and human spermatozoa

    Krasilnikov OV, Merzliak PG, Yuldasheva LN, et al. PROG BIOPHYS MOL BIO 65: PC336-PC336 Suppl. 1 1996
     Are ion channels distributed randomly in a planar lipid bilayer membranes?

    Gessner G, Meder S, Rink T, et al. PESTIC SCI 48: (4) 399-407 DEC 1996
     Ionophore and anthelmintic activity of PF 1022A, a cyclooctadepsipeptide, are not related

    Lin MX, Mirzabekov T, Kagan BL J BIOL CHEM 272: (1) 44-47 JAN 3 1997
     Channel formation by a neurotoxic prion protein fragment

    Hirakura Y, AlvarezBravo J, Kurata S, et al. J BIOCHEM-TOKYO 120: (6) 1130-1140 DEC 1996
     Selective interaction of synthetic antimicrobial peptides derived from sapecin B with lipid bilayers

Denicourt N, Cai S, Garneau L, et al. BBA-BIOMEMBRANES 1285: (2) 155-166 DEC 4 1996
     Evidence from incorporation experiments for an anionic channel of small conductance at the apical membrane of the rabbit distal tubule

    Krasilnikov OV, Carneiro CMM, Yuldasheva LN, et al. BRAZ J MED BIOL RES 29: (12) 1691-1697 DEC 1996
     Diameter of the mammalian porin channel in open and ''closed'' states: Direct measurement at the single channel level in planar lipid bilayer

    Abrahamse SL, Bridges RJ, DeJong MD, et al. ANAL BIOCHEM 242: (1) 112-122 NOV 1 1996
     Apical membrane isolation of surface and crypt cells from rabbit distal colon

    Wada S, Iida A, Asami K, et al. BIOORG MED CHEM LETT 6: (19) 2275-2278 OCT 8 1996
     Ion channel-forming property of trochorovin-XII, an 11-residue peptaibol from the fungus Trichoderma viride, in planar lipid bilayer membranes

    Aistrup GL, Szentirmay M, Kumar KN, et al. FEBS LETT 394: (2) 141-148 SEP 30 1996
     Ion channel properties of a protein complex with characteristics of a glutamate/N-methyl-D-aspartate receptor

    Ludwig A, Garcia F, Bauer S, et al. J BACTERIOL 178: (18) 5422-5430 SEP 1996
     Analysis of the in vivo activation of hemolysin (HlyA) from Escherichia coli

    Fujimoto I, Oiki S, Kondo T, et al. NEUROSCI RES 25: (3) 229-237 JUL 1996
     GTP-binding protein activation underlies LTP induction by mast cell degranulating peptide

    Nagaoka Y, Iida A, Kambara T, et al. BBA-BIOMEMBRANES 1283: (1) 31-36 AUG 14 1996
     Role of proline residue in the channel-forming and catecholamine-releasing activities of the peptaibol,
     trichosporin-B-VIa

    Thrower EC, Duclohier H, Lea EJA, et al. BIOCHEM J 318: 61-66 Part 1 AUG 15 1996
     The inositol 1,4,5-trisphosphate-gated Ca2+ channel: Effect of the protein thiol reagent thimerosal on channel activity

    Lemaitre C, Orange N, Saglio P, et al. EUR J BIOCHEM 240: (1) 143-149 AUG 15 1996
     Characterization and ion channel activities of novel antibacterial proteins from the skin mucosa of carp (Cyprinus carpio)
Wiesner P, Popp B, Schmid A, et al. BBA-BIOMEMBRANES 1282: (2) 216-224 JUL 25 1996
     Isolation of mitochondrial porin of the fly Protophormia: Porin modification by the pesticide CGA 140'408 studied in lipid bilayer membranes

    Salamon Z, Tollin G BIOPHYS J 71: (2) 848-857 AUG 1996
     Surface plasmon resonance studies of complex formation between cytochrome c and bovine cytochrome c oxidase incorporated into a supported planar lipid bilayer .1. Binding of cytochrome c to cardiolipin/phosphatidylcholine membranes in the absence of oxidase

    Salamon Z, Tollin G BIOPHYS J 71: (2) 858-867 AUG 1996
     Surface plasmon resonance studies of complex formation between cytochrome c and bovine cytochrome c oxidase
     incorporated into a supported planar lipid bilayer .2. Binding of cytochrome c to oxidase-containing
     cardiolipin/phosphatidylcholine membranes

    Skare JT, Champion CI, Mirzabekov TA, et al. J BACTERIOL 178: (16) 4909-4918 AUG 1996
     Porin activity of the native and recombinant outer membrane protein Oms28 of Borrelia burgdorferi

    White PJ J MEMBRANE BIOL 152: (1) 89-99 JUL 1996
The permeation of ammonium through a voltage-independent K+ channel in the plasma membrane of rye roots

    Miedema H, Staal H, Prins HBA.,  J MEMBRANE BIOL 152: (2) 159-167 JUL 1996
     pH-induced proton permeability changes of plasma membrane vesicles

    Huang HJ, Hancock REW J BACTERIOL 178: (11) 3085-3090 JUN 1996
     The role of specific surface loop regions in determining the function of the imipenem-specific pore protein OprD of Pseudomonas aeruginosa

    Tripathy A, Meissner G BIOPHYS J 70: (6) 2600-2615 JUN 1996
     Sarcoplasmic reticulum lumenal Ca2+ has access to cytosolic activation and inactivation sites of skeletal muscle Ca2+   release channel

    White PJ J EXP BOT 47: (298) 713-716 MAY 1996
     Specificity of ion channel inhibitors for the maxi cation channel in rye root plasma membranes

    Giffard CJ, Ladha S, Mackie AR, et al. J MEMBRANE BIOL 151: (3) 293-300 JUN 1996
Interaction of nisin with planar lipid bilayers monitored by fluorescence recovery after photobleaching

Nelson TE, Lin M, ZapataSudo G, et al. ANESTHESIOLOGY 84: (6) 1368-1379 JUN 1996
     Dantrolene sodium can increase or attenuate activity of skeletal muscle ryanodine receptor calcium release channel - Clinical implications

    Li CH, Ramjeesingh M, Bear CE.,  J BIOL CHEM 271: (20) 11623-11626 MAY 17 1996
Purified cystic fibrosis transmembrane conductance regulator (CFTR) does not function as an ATP channel

    ShoshanBarmatz V, Hadad N, Feng W, et al. FEBS LETT 386: (2-3) 205-210 MAY 20 1996
     VDAC/porin is present in sarcoplasmic reticulum from skeletal muscle

    Bonhivers M, Ghazi A, Boulanger P, et al. EMBO J 15: (8) 1850-1856 APR 15 1996
     FhuA, a transporter of the Escherichia coli outer membrane, is converted into a channel upon binding of bacteriophage T5

    Zchut S, Feng W, ShoshanBarmatz V BIOCHEM J 315: 377-383 Part 2 APR 15 1996
Ryanodine receptor calcium release channel conformations as reflected in the different effects of propranolol on its ryanodine binding and channel activity

    Wang JP, Needleman DH, Seryshev AB, et al. J BIOL CHEM 271: (14) 8387-8393 APR 5 1996
     Interaction between ryanodine and neomycin binding sites on Ca2+ release channel from skeletal muscle sarcoplasmic reticulum

    Sunstrom NA, Premkumar LS, Premkumar A, et al. J MEMBRANE BIOL 150: (2) 127-132 MAR 1996
 Ion channels formed by NB, an influenza B virus protein

    Lii T, Lee HC, Gleason FK, et al. J MEMBRANE BIOL 150: (1) 27-35 MAR 1996
     Characterization of a large conductance, cation-selective channel from sea urchin eggs that is sensitive to sulfhydryl  reducing agents

    Qiu XQ, Jakes KS, Kienker PK, et al. J GEN PHYSIOL 107: (3) 313-328 MAR 1996
     Major transmembrane movement associated with colicin Ia channel gating

    Niles WD, Silvius JR, Cohen FS J GEN PHYSIOL 107: (3) 329-351 MAR 1996
Resonance energy transfer imaging of phospholipid vesicle interaction with a planar phospholipid membrane - Undulations and attachment sites in the region of calcium-mediated membrane membrane adhesion
Ishii J, Nakae T FEMS MICROBIOL LETT 136: (1) 85-90 FEB 1 1996
     Specific interaction of the protein-D2 porin of Pseudomonas aeruginosa with antibiotics

    Feigin AM, Takemoto JY, Wangspa R, et al. J MEMBRANE BIOL 149: (1) 41-47 JAN 1996
Properties of voltage-gated ion channels formed by syringomycin E in planar lipid bilayers

    ORegan MH, Alix S, Woodbury DJ NEUROSCI LETT 202: (3) 201-203 JAN 5 1996
     Phospholipase A(2)-evoked destabilization of planar lipid membranes

    Grosman C, Reisin IL EXP PARASITOL 81: (4) 546-555 DEC 1995
     Echinococcus granulosus: Partial characterization of the conductive properties of two cation channels from protoscoleces of the ovine strain, reconstituted on planar lipid bilayers

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