Research Interests: 
Gene regulation by vitamin A during  early embryonic development; retinoid metabolism and function; environmental pollution and embryonic development.

Teaching Interests:  
Biochemical and molecular biology aspects of function and metabolism of vitamins; biochemical and molecular basis of the relationship between diet, individual nutrients and cancer; causes and prevention of cancer. 

Courses: 
1. HNF/ANS 937 (3CR) Mineral and Vitamin Nutrition and Metabolism.
2. HNF 840 (3CR) Human Nutrition and Chronic Diseases

Undergraduate Advising:  
Human Nutrition: Nutritional Sciences

Graduate Advising:  Human Nutrition

Selected Publications:

Zhou, H-R., Abouzied, M. and Zile, M.H. Production of a hybridoma cell line secreting retinoic acid specific monoclonal antibody. J. Immunol. Meth. 138, 211-223, 1991.

Zile, M.H. Vitamin A homeostasis endangered by environmental pollutants. Proc. Soc. Exptl. Biol. Med. 201, 141-153, 1992.

Dersch, H. and Zile, M.H. Induction of normal cardiovascular development in the vitamin A-deficient quail embryo by natural retinoids. Dev. Biol. 160,424-433, 1993.

Twal, W., Roze, L. and Zile, M.H. Anti-retinoic acid monoclonal antibody localizes all-trans-retinoic acid in target cells and blocks normal development in early quail embryo. Dev. Biol. 168,225-234, 1995.

Chen, Y., Kostetskii, I., Zile, M.H., and Solursh, M. Comparative study of Msx-1 expression in early normal and vitamin A-deficient avian embryos. J. Exptl. Zool. 272,299-310, 1995.

Xu, X.C.,  Zile, M.H., Lippman, S.M., Lee, J.S., Lee, J.J., Hong, W.K. and Lotan, R. Anti-retinoic acid monoclonal antibody binding to premalignant oral lesions is lower than to normal tissue, is increased after treatment with 13-cis-retinoic acid in vivo, and is related to RAR receptor beta expression. Cancer Res. 55,5507-5511, 1995.

Dong, D. and Zile, M.H. Endogenous retinoids in the early avian embryo. Biochem. Biophys. Res. Communic. 217,1026-1031, 1995.

Kostetskii, I., Linask, K., and Zile, M.H. Vitamin A deficiency and the expression of retinoic acid receptors during early cardiogenesis in quail embryo. Roux's Arch. Dev. Biol. 205, 260-271, 1996.

Tsuda, T., Philp, N., Zile, M.H., and Linask, K.K. Left-right asymmetric localization of flectin in the extracellular matrix during heart looping. Dev. Biol. 173, 39-50, 1996.

Chen, Y-P., Dong, D., Kostetskii, I. and Zile, M.H. Hensen's node from vitamin A-deficient quail embryo induces chick limb bud duplication and retains its normal asymmetric expression of Sonic hedgehog (Shh). Dev. Biol. 173, 256-264, 1996.

Maden, M., Gale, E., Kostetskii, I. and Zile, M. Vitamin A-deficient quail embryos have half a hindbrain and other neural defects. Current Biology 6, 417-426, 1996.

Maden, M., Graham, A., Gale, E., Rollinson, C. and Zile, M. Positional apoptosis during vertebrate CNS development in the absence of endogenous retinoids. Development 124, 2799-2805, 1997.

Twal, W.O. and Zile, M. H. Retinoic acid reverses ethanol -induced cardiovascular abnormalities in quail embryos. Alcoholism: Clin. Exptl. Res. 21, 1137-1143, 1997.

Zile, M. H., Summer, C., Aulerich, R., Bursian, S.J., Tillit, D.E., Giesy, J. and Kubiak, T.J. Retinoids in eggs and embryos of birds fed fish from the Great Lakes. Env. Toxicol. Pharmacol. 3, 277-288, 1997.

Zile, M. H. Vitamin A in embryonic development. An overview. In: Symposium: Functional Metabolism of Vitamin A in Embryonic Development. J. Nutr. 128, 455S-458S, 1998.

Maden, M., Gale, E. and Zile, M. H. The role of vitamin A in the development of the central nervous system. In: Symposium: Functional Metabolism of Vitamin A in Embryonic Development. J. Nutr. 128, 471S-475S, 1998.

Kostetskii, I., Yuan, S.Y., Kostetskaia, E., Linask, K., Blanchet, S., Seleiro, E., Brickell, P., Michaille, J. and Zile, M. H. Initial retinoid reqirement for early avian development coincides with retinoid receptor coexpression in the precardiac fields and induction of normal cardiovascular development. Dev. Dyn. 213, 188-198, 1998.

Kostetskii, I., Jiang, Y., Kostetskaia, E., Yuan, S., Evans, T. and Zile, M. H. Retinoid signaling required for normal heart development regulates GATA-4 in a pathway distinct from cardiomyocyte differentiation. Dev. Biol. 206, 206-218, 1999.

Stratford, T., Logan, C., Zile, M. and Maden, M. Abnormal anteroposterior and dorsoventral patterning of the limb bud in the absence of retinoids. Mech. Dev. 81, 115-125, 1999.

Zile, M. H. Avian embryo as a model for retinoid function in early development. In: Handbook of Experimental Pharmacology. Vol. 139. Retinoids (H. Nau and W. Blaner, Eds. ) Ch. 15, 443-464. Springer Verlag, Heidelberg, 1999.

Gale, E., Zile, M. and Maden, M. Hindbrain respecification in the retinoid deficient quail. Mech. Dev. 88, 43-54, 1999.

DeLuca, F., Uyeda, J.A., Mericq, V., Mancilla, E. E., Yanovski, J.A., Zile, M. H. and Baron, J. Retinoic acid is a potent regulator of growth plate chondrogenesis. Endocrinology 141, 346-535, 2000.

Griffith, M. and Zile, M. H. Retinoic acid, midkine and defects of secondary neurulation. Teratology 62, 123-133, 2000.

Ghatpande, S., Ghatpande, A., Zile, M. and Evans, T. Anterior endoderm is sufficient to rescue foregut apoptosis and heart tube morphogenesis in a embryo lacking retinoic acid. Dev. Biol. 219, 59-70, 2000.

Maden, M., Graham, A., Zile, M. and Gale, E. Abnormalities of somite development in the absence of retinoic acid. Int. J. Dev. Biol. 44, 151-159, 2000.

Zile, M. H., Kostetskii, I., Yuan, S., Kostetskaia, E., St. Amand, T., Chen, Y-P. and Jiang, W. Retinoid signaling is required to complete vertebrate cardiac left-right asymmetry pathway. Dev. Biol. 223, 323-338, 2000.

Zile, M. H. Function of vitamin A in vertebrate embryonic development. J. Nutr. 131, 705-708, 2001.

Schlange, T., Schnipkoweit, I., Andrée, B., Ebert, A., Zile, M. H., Arnold, H-H., and Brand, T. Chick CFC controls Leftyl expression in the embryonic midline and Nodal expression in the lateral plate. Dev. Biol. 234, 376-389, 2001.

Ghatpande, S., Ghatpande, A., Sher, J., Zile, M. H. and Evans, T. Retinoid signaling regulates primitive (yolk sac) hematopoiesis. Blood 99, 2379-2386, 2002.

Halilagic, A., Zile, M. H. and Studer, M. A novel role for retinoids in patterning the avian forebrain during presomite stages. Development 130, 2039-2050, 2003.

Cui, J., Michaille, J-J., Jiang, W. and Zile, M. H. Retinoid receptors and vitamin A deficiency: differential patterns of transcription during early avian development and the rapid induction of RARs by retinoic acid. Dev. Biol. 260, 496-511, 2003.

Romeih, M., Cui, J., Michaille, J-J., Jiang, W., and Zile, M. H. Function of RARg and RARa 2 at the initiation of retinoid signaling is essential for avian embryo survival and for distinct events in cardiac morphogenesis. Dev. Dyn. 228, 697-708, 2003.

Zile, M. H. Vitamin A requirement for  early cardiovascular morphogenesis  specification in the vertebrate embryo:Insights from the avian embryo. Expt. Biol. Med. 229,598-606,2004.
LaRue, A.C., Argraves, W. S., Zile, M.H. and Drake, C. J. Critical role for retinol in the generation/differentiation of angioblasts required for embryonic blood vessel formation. Dev. Dyn. 230, 666-674, 2004.